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Chicken LITAF ELISA KIT (96 T) EA800011

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Chicken LITAF ELISA KIT (96 T) EA800011

Chicken LITAF ELISA KIT (96 T)
Chicken TNF-alpha ELISA Kit
货号：EA800011
规格：96T
品牌：OriGene
价格： ￥3200.00
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EA800011
 Product Data
DescriptionChicken TNF-alpha ELISA Kit
Size96 T
Format96-well strip plate
Assay TypeSolid Phase Sandwich ELISA
Assay Length4 hours
SignalColorimetric
Curve Range17.18-1100 pg/ml
Sample TypeSerum, plasma, Cell culture supernatant
Sample Volume100 uL
SpecificityThis assay recognizes both natural and recombinant Chicken TNF-a
Sensitivity8 pg/mL
ReactivityChicken
InterferenceNo significant interference observed with available related molecules.
Components

  • Microwell Plate - antibody coated 96-well Microplate (8 wells ×12 strips),1 plate

  • Standard - lyophilized,2200pg/ml upon reconstitution,2 vials

  • lyophilized, Biotin-Conjugated antibody,2 vials

  • Concentrated Streptavidin-HRP,1 vial

  • Standard /sample Diluent,1 bottle

  • Biotin-Conjugate antibody Diluent,1 bottle

  • Streptavidin-HRP Diluent,1 bottle

  • 20 x Wash Buffer Concentrate,1 bottle

  • Substrate Solution,1 bottle

  • Stop Solution,1 bottle

  • Plate Cover Seals,4 pieces

BackgroundTNF-α, the prototypical member of the TNF protein superfamily, is a homotrimeric type-II membrane protein . Membrane bound TNF-α is cleaved by the metalloprotease TACE/ADAM17 to generate a soluble homotrimer . Both membrane and soluble forms of TNF-α are biologically active. TNF-α is produced primarily by macrophages, but it is produced also by a broad variety of cell types including lymphoid cells, mast cells, endothelial cells, cardiac myocytes, adipose tissue, fibroblasts, and neuronal tissue . Cellular response to TNF-α is mediated through interaction with receptors TNF-R1 and TNF-R2 and results in activation of pathways that favor both cell survival and apoptosis depending on the cell type and biological context. Activation of kinase pathways (including JNK, ERK (p44/42), p38 MAPK and NF-κB) promotes the survival of cells, while TNF-α mediated activation of caspase-8 leads to programmed cell death . TNF-α plays a key regulatory role in inflammation and host defense against bacterial infection, notably Mycobacterium tuberculosis . TNF-α causes many of the clinical problems associated with autoimmune disorders such as rheumatoid arthritis, ankylosing spondylitis, inflammatory bowel disease, psoriasis, hidradenitis suppurativa and refractory asthma. The role of TNF-α in autoimmunity is underscored by blocking TNF-α action to treat rheumatoid arthritis and Crohn’s disease .
Gene SymbolLITAF


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