德国MN NucleoBond Xtra Midi EF无内毒素质粒中提试剂盒
德国MN NucleoBond Xtra Midi EF无内毒素质粒中提试剂盒
货号:740420.50
规格:50 preps
品牌:德国MN
2nd generation of anion exchanger for time saving endotoxin-free plasmid DNA
| Application | Endotoxin-free plasmid DNA isolation |
| Selling unit | 10 Prep(s), 50 Prep(s) |
| Target | Plasmid DNA |
| CE certified | No, research use only |
| Technology | Anion exchange chromatography |
| Brand | NucleoBond |
| Format | Midi prep |
| Handling | Gravity flow |
| Lysate clarification | Column filter |
| Automated use | Yes, can be automated on the Andrew+ pipetting robot |
| Sample material | Bacteria, E. coli |
| Sample amount | < 200 mL (high copy), < 400 mL (low copy) |
| Vector size | < 300 kbp |
| Typical yield | 500 µg |
| Theoretical binding capacity | 1000 µg |
| Typical purity A260/A280 | 1.8−1.95 |
| Endotoxin level | ≤ 0.05 EU/µg DNA |
| Isopropanol precipitation | Centrifugation, NucleoBond Finalizer, NucleoSnap Finisher Midi, NucleoSpin Finisher Midi |
| Preparation time | 85 min/prep |
| Typical downstream application | Cloning, PCR, Restriction analysis, Sequencing, Transfection of cells |
| Storage temperature | 15–25 °C / 59–77 °F |
| Shelf life (from production) | 39 Month(s) |
| Hazardous material | Yes |